Bradford Protein Assay Kit is a ready-to-use formulation of the popular assay reagent originally described by Bradford in 1976. When mixed with a protein solution, the acidic coomassie-dye reagent changes color from brown to blue in proportion the amount of protein present in the sample. Protein determinations are made by comparison to the color response of protein assay standards, usually prepared as a series of known dilutions of bovine serum albumin (BSA). The kit includes Bradford Protein Assay Reagent and BSA standard. It is compatible with many of the salts, solvents, buffers, reducing chemicals and chelating agents often used in protein samples. Assays can be performed in cuvettes, or microplates.
 

Key Features

• Colorimetric: measure with a standard spectrophotometer or plate reader (595 nm).

• Easy to use: single reagent; no working reagent preparation required.

• Fast: almost immediate color development; add, mix and read results.

• Broad range: detects protein concentration in the range 1 to 1500 µg/ml.

• Flexible: microplate or cuvette format.
 

Application

Protein quantitation in solution
 

Components

1. Bradford assay reagent: 500 ml
2. BSA standard: 10 ml, 2 mg/ml
 

Storage

Store at 2-8 °C and protect from light.