Product Description
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Tubulin beta-3 chain |
Synonyms: | TUBB3, CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, beta-4, tubulin beta 3 class III, β3- tubulin, TUJ-1,TUJ 1, TUJ1, tuj1 |
Immunogen: | Recombinant protein corresponding to Mouse beta III Tubulin |
Uniprot ID: | Q9ERD7 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Cytoskeleton |
Predicted MW. / Observed MW. | 55kDa / 55kDa |
Product Usage Information
Applications | Species | Dilution | Positive Tissue |
WB | Human, Mouse, Rat | 1: 1000-1: 2000 | brain, cerebral cortex |
IHC/IF | Human, Mouse, Rat | 1: 600-1: 1200 | cerebellum |
Background
Cysteine residues in class III β-tubulin are actively involved in regulating ligand interactions and microtubule formation. Proteomic analysis has revealed that many factors bound to these cysteine residues are involved in the oxidative stress and glucose deprivation response.This is particularly interesting in light of the fact that class III β-tubulin first appears in the phylogenetic tree when life emerged from the seas and cells were exposed to atmospheric oxygen.
Images
| WB analysis of beta III Tubulin(GB15139). |
| IF analysis of beta III Tubulin(GB15139)red. Sample: Mouse cerebellum, 4% PFA (G1101) 12-24h. Blocking buffer: Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
