Product Description
| Reactivity: | M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Fast Myosin Skeletal Heavy chain rabbit polyclonal antibody |
Gene Name: | myosin heavy chain 1 |
Synonyms: | Myosin heavy chain 2x, MyHC-2x, Myosin heavy chain IIx/d, MyHC-IIx/d, MYH1 |
Uniprot ID: | |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human MYH1. This antibody detects total fast myosin skeletal heavy chain protein. It does cross-react with MYH2,MYH4,MYH8. |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm |
Predicted MW. | 223 kDa |
Observed MW. | 223 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Mouse, Rat | 1: 500-1: 1000 | skeletal muscle |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 1000 | skeletal muscle |
Background
Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells.
Images
| Western blot analysis of MYH1 (GB112130) at dilution of 1: 1000 |
| Immunofluorescent analysis of paraformaldehyde-fixed mouse skeletal muscle using MYH1 (GB112130, red light) and MYH7 (GB112131, green light) at dilution of 1: 1000 |
| Immunofluorescent analysis of paraformaldehyde-fixed rat skeletal muscle using MYH1 (GB112130, red light) and MYH7 (GB112131, green light) at dilution of 1: 1000 |
| Immunofluorescent analysis of paraformaldehyde-fixed rat skeletal muscle using MYH1 at dilution of 1: 1000 |
Storage
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
