Product Description
| Reactivity: | Mouse, Rat | |
| Applications: | IHC | |
| Host Species: | Rabbit | |
| Clonality: | Polyclonal antibody | |
| Gene Name: | heme oxygenase 1 | |
| Gene Symbol: | Hmox1 | |
| Synonyms: | HO1; HO-1; Hmox; Hemox; Hsp32; D8Wsu38e | |
| Gene ID: | 15368 | P14901 |
| >Uniprot ID: | P14901 | |
| Immunogen: | Recombinant protein corresponding to Mouse HMOX1 | |
| Isotype: | IgG | |
| Purity: | Affinity purification | |
| Subcellular location: | Endoplasmic reticulum | |
| Documents: | Manual-Hmox1 polyclonal antibody |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
IHC | Mouse, Rat | 1: 600-1: 1600 | lung, spleen |
Background
Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.
Images
| Immunohistochemistry of paraffin embedded mouse spleen using HMOX1 (GB11549) at dilution of 1:600 (400x lens) |
| Immunohistochemistry of paraffin embedded rat spleen using HMOX1 (GB11549) at dilution of 1:600 (400x lens) |
| Immunofluorescence of paraffin embedded mouse spleen using HMOX1 (GB11549) at dilution of 1:600 (400x lens) |
| Immunofluorescence of paraffin embedded rat lung using HMOX1 (GB11549) at dilution of 1:600 (400x lens) |
| Immunofluorescence of paraffin embedded rat spleen using HMOX1 (GB11549) at dilution of 1:600 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
